anti p53 ic12  (Cell Signaling Technology Inc)

Journal: eLife

Article Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63

doi: 10.7554/eLife.10528

Figure Lengend Snippet: ( A ) Proteins from muscles were immuno-precipitated with a p63 antibody and then separated on a 10% SDS PAGE gel. Western blot experiment was performed using an antibody against p63 total. Shows pools of proteins from 3 animals at 105d. TBP was used as loading control. ( B ) Proteins (40 µg) from muscles were separated on 10% SDS PAGE gel. Western blot probing was performed with p53 antibody (IC12, 1/2000, Cell Signaling, Danvers, MA) and True Blot (Rockland Immunochemicals, Pottstown, PA) secondary antibody avoiding Ig heavy chain recognition. Tubilin was used as loading control. Graph below shows% of induction relative to the mean of p53 expression level in WT animals normalised with tubulin. DOI: http://dx.doi.org/10.7554/eLife.10528.011

Article Snippet: *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex).

Techniques: SDS Page, Western Blot, Expressing

Journal: eLife

Article Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63

doi: 10.7554/eLife.10528

Figure Lengend Snippet: ( A ) mRNA levels of Trim63 in C2C12 cells following transfection with siRNA control and siRNA directed against p73, p53 and a mix of siRNA against P53, and the TA isoforms of Trp63 and P73 (siMIX). Bars represent means (relative induction versus Ct) with standard deviation (n = 3). *p<0.01. ( B ) mRNA level for TA isoforms of Trp63 , TA isoforms of P73 and P53 in C2C12 cells following transfection with siRNA control and siRNA directed against p63, p73, and p53. Bars represent means (relative induction versus Ct) with standard deviation (n = 3). *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex). Bars correspond to means with SD (n = 3). *p<0.01 as calculated by a one-way ANOVA test followed by a Tukey post-test. DOI: http://dx.doi.org/10.7554/eLife.10528.019

Article Snippet: *p<0.01. ( C, D ) Chromatin immunoprecipitation (ChIP) assay was performed on the Trim63 promoter using RT-qPCR on RE1/2 and RE4. p53 immunoprecipitation (C) was performed using p53 antibody IC12 (Cell Signalling), p73 immunoprecipitation was performed using p73 antibody IMG-259a (Imgenex).

Techniques: Transfection, Standard Deviation, Chromatin Immunoprecipitation, Quantitative RT-PCR, Immunoprecipitation

Journal:

Article Title: Activation-induced cytidine deaminase accelerates clonal evolution in BCR-ABL1 -driven B cell lineage acute lymphoblastic leukemia

doi: 10.1158/0008-5472.CAN-10-1438

Figure Lengend Snippet: A. Shown is the log2 expression ratio for the seven different p53 probe sets in the mouse genome 430 2.0 array for each of the AID+/+ samples (N=3) and AID-/- samples (N=3). B. Heat map of downstream p53 targets, molecules involved in the p53 stabilization and p53 degradation. C. Confirmatory quantitative RT-PCR of Ubqln2 and Cdkn1a mRNA levels in AID-/- mice (percentage of Hprt mRNA levels). For each gene, 5 AID-/- samples and 5 AID+/+ samples from different mice were analyzed. D. Western blot analysis of p53 protein levels. Protein from 6 AID-/- leukemia samples and 6 AID+/+ leukemia samples was extracted and evaluated for p53 expression. The AID-/- outlier by PCA component analysis is designated by an asterisk.

Article Snippet: Western blot Monoclonal antibody against p53 (IC12) was purchased from Cell Signaling Technologies (Danvers, MA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal:

Article Title: Activation-induced cytidine deaminase accelerates clonal evolution in BCR-ABL1 -driven B cell lineage acute lymphoblastic leukemia

doi: 10.1158/0008-5472.CAN-10-1438

Figure Lengend Snippet: A. Shown is the log2 expression ratio for the seven different p53 probe sets in the mouse genome 430 2.0 array for each of the AID+/+ samples (N=3) and AID-/- samples (N=3). B. Heat map of downstream p53 targets, molecules involved in the p53 stabilization and p53 degradation. C. Confirmatory quantitative RT-PCR of Ubqln2 and Cdkn1a mRNA levels in AID-/- mice (percentage of Hprt mRNA levels). For each gene, 5 AID-/- samples and 5 AID+/+ samples from different mice were analyzed. D. Western blot analysis of p53 protein levels. Protein from 6 AID-/- leukemia samples and 6 AID+/+ leukemia samples was extracted and evaluated for p53 expression. The AID-/- outlier by PCA component analysis is designated by an asterisk.

Article Snippet: Monoclonal antibody against p53 (IC12) was purchased from Cell Signaling Technologies (Danvers, MA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot